產品編號 yb-1622M
英文名稱 Anti-AFP(A4)抗體
中文名稱 甲胎蛋白單克隆抗體(檢測)
別    名 Alpha Fetoprotein; Alpha-fetoprotein; AFP; alpha 1 fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin; alpha fetoglobulin; alpha Fetoprotein; Alpha fetoprotein precursor; FETA; HPAFP; FETA_HUMAN.
Anti-AFP(A4)抗體  
說 明 書 0.1ml  0.2ml  
研究領域 腫瘤  
抗體來源 Mouse
克隆類型 Monoclonal
克 隆 號 A4
交叉反應 Human, 
產品應用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 65kDa
細胞定位 分泌型蛋白 
性    狀 Lyophilized or Liquid
濃    度 1mg/1ml
免 疫 原 AFP protein
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Anti-AFP(A4)抗體
PubMed PubMed
產品介紹 background:
Alpha 1 Fetoprotein is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. Expression has been documented in human adrenal, liver, ovary, testis, and pancreas. ESTs have been isolated from normal human brain, liver/spleen, embryo and uterus tissue libraries. 

Function:
Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.

Subunit:
Dimeric and trimeric forms have been found in addition to the monomeric form.

Subcellular Location:
Secreted.

Tissue Specificity:
Plasma. Synthesized by the fetal liver and yolk sac.

Similarity:
Belongs to the ALB/AFP/VDB family. 
Contains 3 albumin domains.

Anti-AFP(A4)抗體是由胚胎卵黃囊細胞，胚胎肝細胞和胎兒腸道細胞合成的一種糖蛋白。主要用于原發性或轉移性肝細胞癌、肝樣胃癌和性腺或性腺外某些生殖細胞腫瘤(如內胚竇瘤)的診斷和鑒別診斷。
AFP敏感度較低，特異性強，大約60%左右的肝細胞肝癌陽性，在低分化腫瘤中常呈陽性表達。
該抗體與bsm-1621M（包被）（Anti-Alpha Fetoprotein）配對使用；主要用于ELISA試劑盒，膠體金試劑條制備生產都很好。