產品編號 yb-1473R
英文名稱 Anti-AKAP95抗體
中文名稱 蛋白激酶A錨定蛋白95抗體
別    名 AKAP8; AKAP-8; AKAP-95; A-kinase Anchoring Protein 95; CCK 1; CCK1; CCL 28; Chemokine (C-C motif) ligand 28; MEC; Mucosae associated epithelial chemokine; SCYA28; Small inducible cytokine A28; AKP8L_HUMAN.
Anti-AKAP95抗體  
說 明 書 0.1ml  0.2ml  
研究領域 細胞生物  信號轉導  細胞凋亡  細胞周期蛋白  激酶和磷酸酶  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Rabbit, 
產品應用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 71kDa
性    狀 Lyophilized or Liquid
濃    度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human AKAP95
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed PubMed
產品介紹 background:
This gene encodes a member of the A-kinase anchor protein family. A-kinase anchor proteins are scaffold proteins that contain a binding domain for the RI/RII subunit of protein kinase A (PKA) and recruit PKA and other signaling molecules to specific subcellular locations. This gene encodes a nuclear A-kinase anchor protein that binds to the RII alpha subunit of PKA and may play a role in chromosome condensation during mitosis by targeting PKA and the condensin complex to chromatin. A pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq, May 2011]

Function:
Could play a role in constitutive transport element (CTE)-mediated gene expression. Does not seem to be implicated in the binding of regulatory subunit II of PKA. May be involved in nuclear envelope breakdown and chromatin condensation. May regulate the initiation phase of DNA replication when associated with TMPO-beta.

Subunit:
Interacts with PRPF40A. Binds to the C-terminal of RNA helicase A and to TMPO-beta. Interacts with RNF43.

Subcellular Location:
Nucleus matrix. Cytoplasm. Note=Colocalizes with PRPF40A in the nuclear matrix. Nuclear at steady state but shuttles between the nucleus and cytoplasm. Associated with the nuclear matrix.

Tissue Specificity:
Expressed in the brain cortex (at protein level).

Post-translational modifications:
Phosphorylated on serine or threonine residues possibly by PKA.

Similarity:
Belongs to the AKAP95 family. 
Contains 2 C2H2 AKAP95-type zinc fingers.


蛋白激酶A錨定蛋白95參與細胞分裂染色體集縮的建立和集縮狀態的保持,參與基因表達調控、DNA復制以及維持mRNA的穩定,參與胚胎發育、細胞凋亡及細胞周期調控等。