產品編號 yb-1114R
英文名稱 Anti-beta Adducin抗體
中文名稱 內收蛋白抗體
別    名 ADD 2; ADD B; ADD2; ADDB; ADDL; Adducin 2 (beta); Adducin 2; Adducin like; Adducin like protein 70; Adducin2; Beta adducin; Erythrocyte adducin alpha subunit; Erythrocyte adducin subunit alpha; Erythrocyte adducin subunit beta; MGC3339; MGC44427; ADDB_HUMAN.
Anti-beta Adducin抗體    
說 明 書 0.1ml  0.2ml  
研究領域 細胞生物  信號轉導  結合蛋白  細胞類型標志物  細胞骨架  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human, Mouse, Rat, Dog, Horse, Rabbit, 
產品應用 WB=1:200-1000 ELISA=1:500-5000 IHC-P=1:200-1000 IHC-F=1:200-1000 IF=1:200-1000 （石蠟切片需做抗原修復） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 80kDa
細胞定位 細胞漿 細胞膜 
性    狀 Lyophilized or Liquid
濃    度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human beta Adducin protein
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed PubMed
產品介紹 background:
Adducins are a family of cytoskeletal proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha and beta adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha and gamma adducins are ubiquitously expressed. In contrast, beta adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca2+/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described.

Function:
Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin. Calmodulin binds preferentially to the beta subunit.

Subunit:
Heterodimer of an alpha and a beta subunit.

Subcellular Location:
Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side.

Tissue Specificity:
Expressed mainly in brain, spleen, kidney cortex and medulla, and heart. Also expressed in human umbilical vein endothelial cells, human vascular smooth muscle cells, kidney tubular cells and K-562 cell line.

Post-translational modifications:
The N-terminus is blocked.

Similarity:
Belongs to the aldolase class II family. Adducinbn subfamily.

內收蛋白(Adducin）是一種膜骨架蛋白，內收蛋白可與肌動蛋白及血影蛋白復合體結合，并且通過Ca2+和鈣調蛋白的作用影響骨架蛋白的穩(wěn)定性，從而影響紅細胞的形態(tài).