產品編號 yb-3371R
英文名稱 Anti-Phospho-A Raf(Ser299)抗體
中文名稱 磷酸化A-Raf抗體
別    名 A RAF (phospho S299); p-A RAF (phospho S299); A RAF (phospho S299); p-A RAF (phospho S299); A raf 1; A Raf proto oncogene serine/threonine protein kinase; ARAF 1; ARAF; ARaf proto oncogene serine/threonine protein kinase; ARAF1; Oncogene Araf1; Oncogene PKS2; PKS 2; PKS; PKS2; Proto oncogene Pks; RAFA 1; RAFA1; Ras binding protein DA Raf; v raf murine sarcoma 3611 viral oncogene homolog; v raf murine sarcoma 3611 viral oncogene homolog 1; v raf oncogene homolog 1 (murine sarcoma 3611 virus); ARAF_HUMAN.
Anti-Phospho-A Raf(Ser299)抗體 
說 明 書 0.1ml  
產品類型 磷酸化抗體 
研究領域 腫瘤  細胞生物  免疫學  信號轉導  細胞凋亡  轉錄調節因子  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, 
產品應用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 67kDa
性    狀 Lyophilized or Liquid
濃    度 1mg/1ml
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human A Raf around the phosphorylation site of Ser299
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Anti-Phospho-A Raf(Ser299)抗體
PubMed PubMed
產品介紹 background:
The RAF family of signaling proteins has three isoforms: A RAF, RAF 1 and B RAF. These proteins are instrumental in forwarding the stimulus generated by mitogens from monomeric G proteins to the MAP kinase pathway. RAF 1 is known to be downstream of Ras and binds to it by an N terminal noncatalytic domain. This domain is conserved in A RAF and B RAF. 14-3-3 is also known to be an activator of RAFs. Activated RAF directly phosphorylates and activates MEK. A RAF is expressed at a very high concentration in urogenital and kidney tissues.

Function:
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus.

Subunit:
Interacts with TH1L/NELFD.

Tissue Specificity:
Predominantly in urogenital tissues.

Similarity:
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
Contains 1 phorbol-ester/DAG-type zinc finger. 
Contains 1 protein kinase domain. 
Contains 1 RBD (Ras-binding) domain.

Anti-Phospho-A Raf(Ser299)抗體
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.