α1-微球蛋白(α1M)多克隆抗體 

Polyclonal Antibody to Alpha-1-Microglobulin/Bikunin Precursor (a1M)

FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!

α1-微球蛋白(α1M)多克隆抗體IMMUNOGEN INFORMATION

Immunogen: Recombinant a1M (Val22~Val203) expressed in E.coli.

ANTIBODY SPECIFITY

The antibody is a rabbit polyclonal antibody raised against a1M. It has been selected for its ability to recognize a1M in immunohistochemical staining and western blotting.

α1-微球蛋白(α1M)多克隆抗體APPLICATIONS

Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

CONTENTS

Form & Buffer: Supplied as solution form in PBS, pH7.4,
containing 0.02% NaN3, 50% glycerol.

IHC Images

Used in DAB staining on fromalin fixed paraffin- embedded liver tissue

α1-微球蛋白(α1M)多克隆抗體QUALITY CONTROL

Content: The quality control contains recombinant a1M (Val22~Val203) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
                5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.

STORAGE

Store at 4^oC for frequent use. Stored at -20^oC to -80^oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.